Bismuth Sulfite Agar (Wilson and Blair
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Italy
Known as an Italian recipe, with similar versions prepared across other nations.
Ingredients 2 ingredients
- 20 g Distilled water
- 10 g Beef
Cook safely
Keep children and pets away from the hob and the oven, and keep oven gloves and a lid within reach.
- Boiling water and steam. Turn pan handles inward, away from the edge of the hob. Lift lids away from you, and pour hot water away from your body: steam burns too.
Method
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- Polypeptone (or peptone) 10 g Beef extract 5 g Dextrose 5 g NajHP0 4 (anhydrous) 4 g FeSO t (ahnydrous) 0.3 g Bismuth sulfite (indicator) 8 g Brilliant green 0.025 g Agar 20 g Distilled water 1 litre Mix thoroughly and heat with agitation.
- Boil about 1 min to obtain uniform suspension.
- (Precipitate will not dissolve.) Cool to 45-50*.
- Suspend precipitate by gentle agitation, and pour 20 ml portions into sterile 15 x 100 mm petri dishes.
- Let plates dry about 2 h with lids partially removed;
- then close plates.
- Final pH, 7.6 ± 0.2.
- Do not autoclave .
- Prepare plates on day before streaking and store in dark.
- Selectivity decreases in 48 h.
Plating
Cookware for this recipe
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Saucepan