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Purple Carbohydrate Broth

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Italy

Known as an Italian recipe, with similar versions prepared across other nations.

Ingredients 3 ingredients

  1. 02 g Distilled water
  2. Broth
  3. 10 g Beef

Cook safely

Keep children and pets away from the hob and the oven, and keep oven gloves and a lid within reach.

  • Boiling water and steam. Turn pan handles inward, away from the edge of the hob. Lift lids away from you, and pour hot water away from your body: steam burns too.

Method

  1. Proteose peptone No.
  2. 3 10 g Beef extract (optional) 1 g NaCl 5 g Bromcresol purple 0.02 g Distilled water 1 litre Prepare as for phenol red carbohydrate broth (M109) .
  3. Final pH, 6.8 ± Selenite Cystine Broth Medium 1 Tryptone or polypeptone 5 g lactose 4 g Sodium selenite (NaHSeOj) 4 g Na 2 HP0 4 10 g L-Cystine 0.01 g Distilled water 1 litre Heat to boiling to dissolve.
  4. Dispense 10 ml portions into sterile 16 x 150 mm test tubes.
  5. Heat 10 min in flowing steam.
  6. Do not autoclave .
  7. Final pH, 7.0 ± 0.2.
  8. The medium is not sterile.
  9. Use same day as prepared.
  10. Medium 2 l North- Ba rtram modification^ Polypeptone 5 g Lactose 4 g Sodium selenite (NaHSeOj) 4 g NajHP0 4 5.5 g KHjP 0 4 4.5 g Lr-Cy stine 0.01 g Distilled water 1 litre Heat with agitation to dissolve.
  11. Dispense 10 ml portions to sterile 16 x 150 mn test tubes.
  12. Heat 10 min in flowing steam.
  13. Do not autoclave .
  14. Use same day as prepared.

Plating

Cookware for this recipe

  • Saucepan