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Sintnons Citrate Agar

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Italy

Known as an Italian recipe, with similar versions prepared across other nations.

Ingredients 4 ingredients

  1. 1 litre distilled water
  2. Broth
  3. Salts
  4. 15 g Distilled water

Cook safely

Keep children and pets away from the hob and the oven, and keep oven gloves and a lid within reach.

  • Boiling water and steam. Turn pan handles inward, away from the edge of the hob. Lift lids away from you, and pour hot water away from your body: steam burns too.

Method

  1. Sodium citrate -2^0 2 g NaCl 5 g iyiP0 4 1 g NiyLjPq.
  2. 1 g Mgso 4 0.2 g Bromthymol blue 0.08 g Agar 15 g Distilled water 1 litre Heat gently with occasional agitation.
  3. Boil 1-2 min until agar dissolves.
  4. Fill 13 x 100 or 16 x 150 mm screw-cap tubes 1/3 full.
  5. Autoclave 15 min at 121” .
  6. Before medium solidifies, incline tubes to obtain 4-5 cm slants and 2-3 cm butts.
  7. Final pH, 6.9 ± 0.2.
  8. Tetrathionate Broth Tetrathionate broth base Polypeptone 5 g Bile salts 1 g Calcium carbonate 10 g Sodium thiosulfate -SHjO 30 g Distilled water 1 litre Suspend ingredients in 1 litre distilled water, mix, and heat to boiling.
  9. (Precipitate will not dissolve completely.) Cool to less than 45*.
  10. Store at 5-8*.
  11. Final pH, 8.4 ± 0.2.
  12. Iodine-Potassium Iodide (I-Kt) solution Potassium iodide 5 g Iodine, resublimed 6 g Distilled water, sterile 20 ml Dissolve potassium iodide en 5 ml sterile distilled water.
  13. Add iodine and stir to dissolve.
  14. Dilute to 20 ml.
  15. Brilliant green solution Brilliant green dye, sterile Distilled water, sterile On day of use, , add 20 ml I-KT solution and 10 ml brilliant green solution to 1 litre base.
  16. Resuspend precipitate by gentle agitation and aseptically dispense 10 ml portions into 20 x 150 or 16 x 150 mm sterile test tubes.
  17. Do not heat medium after addition of I-KT and dye solutions.

Plating

Cookware for this recipe

  • Saucepan
  • Wooden spoon