Toluldine Blue-DNA Agar
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Italy
Known as an Italian recipe, with similar versions prepared across other nations.
Ingredients 2 ingredients
- 1 liter distilled water
- 1 g Distilled water
Cook safely
Keep children and pets away from the hob and the oven, and keep oven gloves and a lid within reach.
- Boiling water and steam. Turn pan handles inward, away from the edge of the hob. Lift lids away from you, and pour hot water away from your body: steam burns too.
Method
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- Deoxyribonucleic acid (DNA): 0.3 g Agar: 10 g CaCI 2 (anhydrous): 1.1 mg NaCI: 10 g Toluidine blue O: 83 mg Tris (hydroxymethyl) aminomethane: 6.1 g Distilled water: 1 litre Dissolve Tris (hydroxymethyl) aminomethane in 1 liter distilled water.
- Adjust pH to 9.0.
- Add the remaining ingredients except toluidine blue O and heat to boiling to dissolve.
- Dissolve toluidine blue O in medium.
- Dispense to rubber-stoppered flasks.
- Sterilization is not necessary if used immediately.
- The sterile medium is stable at room temperature for 4 months and is satisfactory after several melting cycles.
Plating
Cookware for this recipe
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Saucepan