Baird-Parker Medium
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Italy
Known as an Italian recipe, with similar versions prepared across other nations.
Ingredients 4 ingredients
- 10 g Beef
- 20 g Distilled water
- Soy
- 5 g Yeast
Contains
Soy
Read from the ingredients, not verified. Check labels.
Cook safely
Keep children and pets away from the hob and the oven, and keep oven gloves and a lid within reach.
- Boiling water and steam. Turn pan handles inward, away from the edge of the hob. Lift lids away from you, and pour hot water away from your body: steam burns too.
Method
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- Tryptone: 10 g Beef extract: 5 g Yeast extract: 1 g Sodium pyruvate: 10 g Glycine: 12 g Lithium chloride 6H 2 0: 5 g Agar: 20 g Distilled water: 1 litre Autoclave 15 min at 121“.
- Final pH, 7.0 ± 0.2.
- If desired for immediate use, maintain melted medium at 48-50“ before adding enrichment.
- Otherwise, store solidified medium at 4 ± 1“ up to 1 month.
- Melt medium before use.
- Trypticase (Tryptic) Soy Agar Trypticase peptone: 15 g Phytone peptone: 5 g NaCI: 5 g Agar: 15 g Distilled water: 1 litre Heat with agitation to dissolve agar.
- Boil 1 min.
- Dispense into suitable tubes or flasks.
- Autoclave 15 min at 121°.
- Final pH.
- 7.3 ± 0.2.
Plating
Cookware for this recipe
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Saucepan