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Trvpticase Sov-Trvptose Broth

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Italy

Known as an Italian recipe, with similar versions prepared across other nations.

Ingredients 5 ingredients

  1. 1 litre water
  2. 3 g Distilled water
  3. 5 g Yeast
  4. Broth
  5. Soy
Contains Soy Read from the ingredients, not verified. Check labels.

Method

  1. Trypticase soy broth (cormercial , dehydrated) 15 g Tryptose broth (contnercial , dehydrated) 13.5 g Yeast extract 3 g Distilled water 1 litre Dissolve ingredients in 1 litre water.
  2. Heat gently to dissolve.
  3. Dispense 5 ml portions into 16 x 150 mm test tubes.
  4. Autoclave 15 min at 121“ .
  5. Final pH, 7.2 ± 0.2.
  6. Tryptone f Tryptophane) Broth.
  7. 1% Tryptone or trypticase Distilled water 1 Dissolve and dispense 5 ml portions into 16 tubes.
  8. Autoclave 15 min at 121*.
  9. Final pH, y.
  10. oarahaemolvticus .
  11. add 30 g NaCl.

Plating